Team:Brown-Stanford/Lab/Protocols/Bang

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Brown-Stanford
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Protocol Code: M7

Bang Media

By Percentage

  • 1% tryptone
  • 0.5% yeast extract
  • 0.45 tricine
  • 0.5% (NH4)2SO4
  • 0.2% glutamic acid
  • 1% urea

By Mass per Liter

  • 10g tryptone
  • 5g yeast extract
  • 4.5g tricine
  • 5g (NH4)2SO4
  • 2g glutamic acid
  • 10g urea
  • Optional: 15g agar (for plates)

Notes

  • Mix in 200mL of water (without agar) to make a 5x solution.
  • Needs to be at a pH of 8.5

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