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Team:British Columbia/Week7
From 2011.igem.org
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Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes. | Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes. | ||
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Daisy did a DpnI digest of her multiple site-directed mutagenesis product and ran it on a gel. The appropriate sizes were shown on the gel, running at 7.6 kb. Daisy transformed the products into Dh5 alpha cells. | Daisy did a DpnI digest of her multiple site-directed mutagenesis product and ran it on a gel. The appropriate sizes were shown on the gel, running at 7.6 kb. Daisy transformed the products into Dh5 alpha cells. | ||
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Latest revision as of 07:33, 30 June 2011
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Week 7: Monday June 13 - Sunday June 19
Contents |
Monday
Daisy is working with the synthase 3-carene; 3-carene has three restriction sites that need to be modified (EcoRI, XbaI, PstI). She worked over the weekend, and has one more site to modify.
We learned that the truncated version of the synthase will increase the efficiency of the enzyme.
Tuesday
Wednesday
Canucks lost to the Bruins. It was a sad day for all..
Thursday
Friday
Saturday
3-Carene
Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes.
Sunday
3-Carene
Daisy did a DpnI digest of her multiple site-directed mutagenesis product and ran it on a gel. The appropriate sizes were shown on the gel, running at 7.6 kb. Daisy transformed the products into Dh5 alpha cells.
