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Team:Amsterdam/Project/Data
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| - | ==Data for our favorite new parts== | + | ===Data for our favorite new parts=== |
| - | + | #[http://partsregistry.org/wiki/index.php?title=Part:BBa_K538004 Cspc]: this chaperone has been confirmed to increase survival rate of ''E. coli'' after multiple freeze/thaw cycles when expressing this cold shock protein. | |
| - | + | #[http://partsregistry.org/Part:BBa_K538000 Cpn10]: Experiments carried out on this gene suggests a possible increase in the survival rate of ''E. coli'' after multiple freeze/thaw cycles when expressing this chaperone. Further research is needed to validate these results. | |
| - | ==Data for existing parts== | + | ===Data for existing parts=== |
| - | + | #[http://partsregistry.org/Part:BBa_R0010:Experience R0010]: This promoter is always active in Top 10 ‘’E. coli’’, because it lacks the LacI protein. Our findings seem to confirm this when used in combination with CspC. | |
| - | + | #[http://partsregistry.org/Part:BBa_B0034:Experience B0034]: Going by previous experience with this RBS we chose it on the grounds of the positive comments shown on the experience page. Our results show proteins can be expressed at low temperatures with this RBS in front of them. From this we conclude that the RBS is also useful for translation in cold environments. | |
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} | ||
Revision as of 23:12, 21 September 2011
Data
Data for our favorite new parts
- Cspc: this chaperone has been confirmed to increase survival rate of E. coli after multiple freeze/thaw cycles when expressing this cold shock protein.
- Cpn10: Experiments carried out on this gene suggests a possible increase in the survival rate of E. coli after multiple freeze/thaw cycles when expressing this chaperone. Further research is needed to validate these results.
Data for existing parts
- R0010: This promoter is always active in Top 10 ‘’E. coli’’, because it lacks the LacI protein. Our findings seem to confirm this when used in combination with CspC.
- B0034: Going by previous experience with this RBS we chose it on the grounds of the positive comments shown on the experience page. Our results show proteins can be expressed at low temperatures with this RBS in front of them. From this we conclude that the RBS is also useful for translation in cold environments.
