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Team:Amsterdam/Labwork/Characterization
From 2011.igem.org
Contents |
Characterization
So in the end, we got two working assembly’s.
Cpn10
The first is a construct with lacI, strong RBS and Cpn10. In our E.coli lacI is always present so the promoter is always on. Cpn10 was selected, because it is a chaperone that’s works together with Cpn60 and helps the folding of proteins at lower temperatures. We do not expect any influence of Cpn10 on the growth curves, because it is the smaller one of the two chaperones. Unfortunately we didn’t get Cpn60 in a construct.
CspC
The second construct is lacI, strong RBS and CspC. CspC is known for its positive influence on survival rate in a Freeze/thaw cycle. There is no indication that CspC has an effect on the growth rate at lower temperatures.
Growth curves
[under construction]
We expected that if our genes would have any effect, it would be a higher specific growth rate at low temperatures (4 to 30°C), and no effect on the specific growth rate at 37°C (the optimal temperature for E.coli). Figure 1 shows that Cpn10 and CspC do not contribute to a higher specific growth rate at lower temperatures.
