Team:Amsterdam/8 September 2011

From 2011.igem.org

Revision as of 08:33, 21 September 2011 by Jorickm (Talk | contribs)

April
MTWTFSS
        [http://2011.igem.org/Team:Amsterdam/1_April_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_April_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_April_2011&action=edit 3]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_April_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_April_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_April_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_April_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_April_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_April_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_April_2011&action=edit 10]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_April_2011&action=edit 11] [http://2011.igem.org/Team:Amsterdam/12_April_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_April_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_April_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_April_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_April_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_April_2011&action=edit 17]
[http://2011.igem.org/Team:Amsterdam/18_April_2011 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_April_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_April_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_April_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_April_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_April_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_April_2011&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_April_2011&action=edit 25] [http://2011.igem.org/Team:Amsterdam/26_April_2011 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_April_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_April_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_April_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_April_2011&action=edit 30]
May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_May_2011&action=edit 1]
[http://2011.igem.org/Team:Amsterdam/2_May_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_May_2011&action=edit 8]
[http://2011.igem.org/Team:Amsterdam/9_May_2011 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_May_2011&action=edit 10] [http://2011.igem.org/Team:Amsterdam/11_May_2011 11] [http://2011.igem.org/Team:Amsterdam/12_May_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_May_2011&action=edit 15]
[http://2011.igem.org/Team:Amsterdam/16_May_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_May_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_May_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_May_2011&action=edit 22]
[http://2011.igem.org/Team:Amsterdam/23_May_2011 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_May_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_May_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_May_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_May_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_May_2011&action=edit 29]
[http://2011.igem.org/Team:Amsterdam/30_May_2011 30] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_May_2011&action=edit 31]
June
MTWTFSS
    [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_June_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_June_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_June_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_June_2011&action=edit 5]
[http://2011.igem.org/Team:Amsterdam/6_June_2011 6] [http://2011.igem.org/Team:Amsterdam/7_June_2011 7] [http://2011.igem.org/Team:Amsterdam/8_June_2011 8] [http://2011.igem.org/Team:Amsterdam/9_June_2011 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_June_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/12_June_2011&action=edit 12]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_June_2011&action=edit 13] [http://2011.igem.org/Team:Amsterdam/14_June_2011 14] [http://2011.igem.org/Team:Amsterdam/15_June_2011 15] [http://2011.igem.org/Team:Amsterdam/16_June_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_June_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_June_2011&action=edit 19]
[http://2011.igem.org/Team:Amsterdam/20_June_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_June_2011&action=edit 21] [http://2011.igem.org/Team:Amsterdam/22_June_2011 22] [http://2011.igem.org/Team:Amsterdam/23_June_2011 23] [http://2011.igem.org/Team:Amsterdam/24_June_2011 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_June_2011&action=edit 26]
[http://2011.igem.org/Team:Amsterdam/27_June_2011 27] [http://2011.igem.org/Team:Amsterdam/28_June_2011 28] [http://2011.igem.org/Team:Amsterdam/29_June_2011 29] [http://2011.igem.org/Team:Amsterdam/30_June_2011 30]
July
MTWTFSS
        [http://2011.igem.org/Team:Amsterdam/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_July_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_July_2011&action=edit 3]
[http://2011.igem.org/Team:Amsterdam/4_July_2011 4] [http://2011.igem.org/Team:Amsterdam/5_July_2011 5] [http://2011.igem.org/Team:Amsterdam/6_July_2011 6] [http://2011.igem.org/Team:Amsterdam/7_July_2011 7] [http://2011.igem.org/Team:Amsterdam/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_July_2011&action=edit 10]
[http://2011.igem.org/Team:Amsterdam/11_July_2011 11] [http://2011.igem.org/Team:Amsterdam/12_July_2011 12] [http://2011.igem.org/Team:Amsterdam/13_July_2011 13] [http://2011.igem.org/Team:Amsterdam/14_July_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_July_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_July_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_July_2011&action=edit 17]
[http://2011.igem.org/Team:Amsterdam/18_July_2011 18] [http://2011.igem.org/Team:Amsterdam/19_July_2011 19] [http://2011.igem.org/Team:Amsterdam/20_July_2011 20] [http://2011.igem.org/Team:Amsterdam/21_July_2011 21] [http://2011.igem.org/Team:Amsterdam/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_July_2011&action=edit 24]
[http://2011.igem.org/Team:Amsterdam/25_July_2011 25] [http://2011.igem.org/Team:Amsterdam/26_July_2011 26] [http://2011.igem.org/Team:Amsterdam/27_July_2011 27] [http://2011.igem.org/Team:Amsterdam/28_July_2011 28] [http://2011.igem.org/Team:Amsterdam/29_July_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_August_2011&action=edit 1] [http://2011.igem.org/Team:Amsterdam/2_August_2011 2] [http://2011.igem.org/Team:Amsterdam/3_August_2011 3] [http://2011.igem.org/Team:Amsterdam/4_August_2011 4] [http://2011.igem.org/Team:Amsterdam/5_August_2011 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_August_2011&action=edit 7]
[http://2011.igem.org/Team:Amsterdam/8_August_2011 8] [http://2011.igem.org/Team:Amsterdam/9_August_2011 9] [http://2011.igem.org/Team:Amsterdam/10_August_2011 10] [http://2011.igem.org/Team:Amsterdam/11_August_2011 11] [http://2011.igem.org/Team:Amsterdam/12_August_2011 12] [http://2011.igem.org/Team:Amsterdam/13_August_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_August_2011&action=edit 14]
[http://2011.igem.org/Team:Amsterdam/15_August_2011 15] [http://2011.igem.org/Team:Amsterdam/16_August_2011 16] [http://2011.igem.org/Team:Amsterdam/17_August_2011 17] [http://2011.igem.org/Team:Amsterdam/18_August_2011 18] [http://2011.igem.org/Team:Amsterdam/19_August_2011 19] [http://2011.igem.org/Team:Amsterdam/20_August_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_August_2011&action=edit 21]
[http://2011.igem.org/Team:Amsterdam/22_August_2011 22] [http://2011.igem.org/Team:Amsterdam/23_August_2011 23] [http://2011.igem.org/Team:Amsterdam/24_August_2011 24] [http://2011.igem.org/Team:Amsterdam/25_August_2011 25] [http://2011.igem.org/Team:Amsterdam/26_August_2011 26] [http://2011.igem.org/Team:Amsterdam/27_August_2011 27] [http://2011.igem.org/Team:Amsterdam/28_August_2011 28]
[http://2011.igem.org/Team:Amsterdam/29_August_2011 29] [http://2011.igem.org/Team:Amsterdam/30_August_2011 30] [http://2011.igem.org/Team:Amsterdam/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:Amsterdam/1_September_2011 1] [http://2011.igem.org/Team:Amsterdam/2_September_2011 2] [http://2011.igem.org/Team:Amsterdam/3_September_2011 3] [http://2011.igem.org/Team:Amsterdam/4_September_2011 4]
[http://2011.igem.org/Team:Amsterdam/5_September_2011 5] [http://2011.igem.org/Team:Amsterdam/6_September_2011 6] [http://2011.igem.org/Team:Amsterdam/7_September_2011 7] [http://2011.igem.org/Team:Amsterdam/8_September_2011 8] [http://2011.igem.org/Team:Amsterdam/9_September_2011 9] [http://2011.igem.org/Team:Amsterdam/10_September_2011 10] [http://2011.igem.org/Team:Amsterdam/11_September_2011 11]
[http://2011.igem.org/Team:Amsterdam/12_September_2011 12] [http://2011.igem.org/Team:Amsterdam/13_September_2011 13] [http://2011.igem.org/Team:Amsterdam/14_September_2011 14] [http://2011.igem.org/Team:Amsterdam/15_September_2011 15] [http://2011.igem.org/Team:Amsterdam/16_September_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_September_2011&action=edit 17] [http://2011.igem.org/Team:Amsterdam/18_September_2011 18]
[http://2011.igem.org/Team:Amsterdam/19_September_2011 19] [http://2011.igem.org/Team:Amsterdam/20_September_2011 20] [http://2011.igem.org/Team:Amsterdam/21_September_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_September_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_September_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_September_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_September_2011&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_September_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_September_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_September_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_September_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_October_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_October_2011&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_October_2011&action=edit 31]


08-09-2011

Constructs

The following parts were miniprepped:

  • Cpn10
  • Cpn60
  • CspC
  • K538100
  • K538101
  • K538104
  • K538120
  • K538130
  • K538200
  • Shednak
  • R0010
  • K206000

Digestion

Constructs making using the 2a assembly method. Digestion of different parts to construct the following assemblies:

  • R0010+ B0034 + CspC
  • R0010+ B0034 + Cpn60
  • R0010+ B0034 + Shednak
  • K206000+ B0034 + CspC
  • K206000+ B0034 + Cpn60
  • K206000+ B0034 + Shednak

These assemblies were ligated ON at 16°C.

Backbone transferring

Digestion of different parts to tranfer to the Chloramphenicol backbone:

  • All genes
  • The constructs we've miniprepped today

Gel purification

The different parts were put on gel and purified with the qiaquick gel extraction kit. Afterwards the concentration of the parts were checked on gel. The following parts for the new assembly showed a good result:

  • K538130
  • K206000
  • K538101

K5389120 The following parts for the backbone transferring showed a good result:

  • Cpn60
  • K538200
  • K538101

K538200 Checking

The assembly was checked on gel after digestion with:

  • PvuI + PvuII
  • PvuI + HindIII

On gel this gave the right band sizes.

Retrieved from "http://2011.igem.org/Team:Amsterdam/8_September_2011"