Revision as of 09:57, 27 June 2011 by Jorickm (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

• Home
• Team
  • Students
  • Advisors
  • Lab Environment
• Project
  • Description
  • Modules
  • Data Page
  • Safety
  • Applications
  • Attributions
  • Acknowledgements
• BioBricks
  • Overview
  • Basic Parts
  • Composite Parts
• Labwork
  • Notebook
  • Protocols
  • Characterization
  • Data Analysis
• Human Outreach
  • Collaboration
  • Education
  • Media
• Sponsors
  • Companies
  • Help Us

VU Meeting for topics discussion

Ethylene producing E.coli

• The project is to short
• Are there alternative ways to meassure ethylene?
• Use colors to check if ethylene is produced
• Ethylene sensing is difficult, because of the receptor.
• Evolution doesn't create biobricks

Neglected disease

Kala-azar

• Is the protein available?
• Whole parasite is a problem
• Signal transduction via peanut agglutin can't be done
  • check if peanut agglutin binds to other stuff in human body fluids
• We need specialists on this subject
• Look at Imperial college, they've done a project on parasites
  • Target was an excreted protein

Hookworm

• CCL11 will be cut and luciferase fades when the hookworm is in the sample
  • Can't we do this the other way around
• Metalloproteases cleaves the CCl11, if this isn't available it doesn't promote luciferase production
• Can this be done in E.coli
• Are CCL11 and metalloproteases available for in the lab?

Buruli ulcer

• It is a bacteria
• It is checked with FRET
• Can the specific receptor be palced in E.coli
• The pathogen is known, but no detection method yet
• Is mycolactone detactable?
• Membrane receptor can be a problem
• Maybe use Mycobacteria