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Agenda: Transforming Top 10 cells to Establish Hg sensitive promoter part K346002
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| + | ===''' 05/26/11 Agenda: Transforming Top 10 cells to Establish Hg sensitive promoter part K346002'''=== | ||
| + | (1) 5 microliters of transformation media (BIO RAD Catalogue 166-0409 received 1-17-11 GT) | ||
| + | added to 40 microliters of competent cells in LB broth. | ||
| + | (2) 2 microliters of K346002 DNA added to broth. | ||
| - | | style="color:#008080;background-color:#7CFC00;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | + | (3) Solution was placed in ice bath for 5 minutes. |
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| + | (4) Placed in 44.7 degree Celsius water for 50 seconds. | ||
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| + | (5) Placed in ice bath for 5 minutes. | ||
| + | |||
| + | (6) 1 microliter of SOB buffer at rrom temperature added to broth | ||
| + | (in order to close plasmid channels). | ||
| + | |||
| + | (7) PLated 100 microliters of cells on LB AMP plates (5-26-11 AM). | ||
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| + | Expected: 10 to 50 colonies projected to grow. | ||
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| + | {| style="color:#008080;background-color:#7CFC00;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
!align="center"|[[Team:SouthBend-Mishawaka-HS-2|Home]] | !align="center"|[[Team:SouthBend-Mishawaka-HS-2|Home]] | ||
!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Team|Team]] | !align="center"|[[Team:SouthBend-Mishawaka-HS-2/Team|Team]] | ||
Latest revision as of 16:47, 4 June 2011
05/26/11 Agenda: Transforming Top 10 cells to Establish Hg sensitive promoter part K346002
(1) 5 microliters of transformation media (BIO RAD Catalogue 166-0409 received 1-17-11 GT)
added to 40 microliters of competent cells in LB broth.
(2) 2 microliters of K346002 DNA added to broth.
(3) Solution was placed in ice bath for 5 minutes.
(4) Placed in 44.7 degree Celsius water for 50 seconds.
(5) Placed in ice bath for 5 minutes.
(6) 1 microliter of SOB buffer at rrom temperature added to broth (in order to close plasmid channels).
(7) PLated 100 microliters of cells on LB AMP plates (5-26-11 AM).
Expected: 10 to 50 colonies projected to grow.
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