"

From 2011.igem.org

05/26/11 Agenda: Transforming Top 10 cells to Establish Hg sensitive promoter part K346002

(1) 5 microliters of transformation media (BIO RAD Catalogue 166-0409 received 1-17-11 GT) 
    added to 40 microliters of competent cells in LB broth.

(2) 2 microliters of K346002 DNA added to broth.

(3) Solution was placed in ice bath for 5 minutes.

(4) Placed in 44.7 degree Celsius water for 50 seconds.

(5) Placed in ice bath for 5 minutes.

(6) 1 microliter of SOB buffer at rrom temperature added to broth 
   (in order to close plasmid channels). 

(7) PLated 100 microliters of cells on LB AMP plates (5-26-11 AM). 

Expected: 10 to 50 colonies projected to grow.

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions