Agenda: Establishment of the K346002 Hg Sensitive Promotor

(Difference between revisions)

Latest revision as of 16:42, 4 June 2011

(1)Electroporation of K346002 into Top 10 cells.

(2)40 microliters of Top 10 cells (JH 5-23-10)10E9 + 2 microliters K346002 DNA from Registry.

(3)Parameters of electroporation: not recorded.

(4)Added 1 microliter of SOB media (JH 2-11-11)immediately after electroporation.

(5)Incubated tube at 37 degrees Celsius for 30 minutes.

(6)Plated 200 microliters of transformation mixture on LB Agar AMP 5 micrograms/microliter 
  (4-21-10).Stored mixture at 4 degrees Celsius.

 Expected: 10 to 50 colonies projected to grow.

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

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    Expected: 10 to 50 colonies projected to grow.
+{| style="color:#008080;background-color:#7CFC00;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2|Home]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=SouthBend-Mishawaka-HS-2 Official Team Profile]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Project|Project]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Modeling|Modeling]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Notebook|Notebook]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Safety|Safety]]
+!align="center"|[[Team:SouthBend-Mishawaka-HS-2/Attributions|Attributions]]
+|}