Agenda: Establishment of the K346002 Hg Sensitive Promotor

(1)Electroporation of K346002 into Top 10 cells.

(2)40 microliters of Top 10 cells (JH 5-23-10)10E9 + 2 microliters K346002 DNA from Registry.

(3)Parameters of electroporation: not recorded.

(4)Added 1 microliter of SOB media (JH 2-11-11)immediately after electroporation.

(5)Incubated tube at 37 degrees Celsius for 30 minutes.

(6)Plated 200 microliters of transformation mixture on LB Agar AMP 5 micrograms/microliter 
  (4-21-10).Stored mixture at 4 degrees Celsius.

 Expected: 10 to 50 colonies projected to grow.

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