Team:British Columbia/Notebook/6 July 2011

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Team: British Columbia - 2011.igem.org

beta-pinene

  • Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful.

(-)-limonene

  • Gel verification of the SDM product shows some lanes with smears, and another with 2 bands suggesting unspecified annealing of primers. SDM failed.
  • Troubleshooting: DMSO could be lowering the Tm of the primers; Repeat the SDM PCR without adding DMSO for one sample, without adding MgCl2 for another sample. Use the ramping cycling condition previously indicated.
    • Gel verification of the SDM product shows no bands. Addition of DMSO and MgCl2 is not the problem.
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