Monoterpene Synthase Structural Modeling
Objective: To identify amino acid residues that are terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.
Model Creators: Joe Ho, Samuel Wu
Model Advisors: Shing Hei Zhan, Alina Chan
Three-dimensional structure of the 3 terpene synthases
Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified an experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template. The image was taken using the freely available ICM Browser.
Reactive site of the three synthases
Magnesium ions are cofactors located at the reactive site.
Future Directions
References
1. N. Eswar, M. A. Marti-Renom, B. Webb, M. S. Madhusudhan, D. Eramian, M. Shen, U. Pieper, A. Sali. Comparative Protein Structure Modeling With MODELLER. Current Protocols in Bioinformatics, John Wiley & Sons, Inc., Supplement 15, 5.6.1-5.6.30, 2006.
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Magnesium ions are cofactors located at the reactive site.
