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Team:Amsterdam
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iGEM Team Amsterdam 2011
“Make biology simple with genetically engineered machines”
icE.coli
Escherichia coli's optimal growth temperature is 37°C. Its growth rate decreases sharply at temperatures deviating from this optimum, and growth beneath 8°C is impossible for wildtype E. coli. The aim of the project is to increase the cold tolerance of E. coli, effectively extending the growth curve as shown below. This will be attempted by expressing several different (combinations of) synthesized genes, through standardized plasmids. Succesfully manipulating E. coli's cold tolerance is valuable to both fundamental and applied science. Among others, it enhances the possibilities of heterologous protein expression, and it might lead towards more efficient biosynthesis of various heat-sensitive compounds (such as antibiotics).Updates
- 22/09/11 - Wikifreeze: All of our pages have been finalized!
- 08/09/11 - The BioBricks pages are undergoing a major overhaul!
- 06/09/11 - Over the last weeks all pages have received updates. Be sure to check them all!
- 10/08/11 - The Description has been updated!
- 27/07/11 - The Notebook page has been restructured!
- 26/07/11 - The Students page has been updated!
- 19/07/11 - Several meetings and lab journal entries placed onto notebook
- 13/07/11 - The Project Description and Safety pages have been updated!
- 08/07/11 - Several meetings placed onto notebook
- 06/07/11 - Added several lab journal entries
- 05/07/11 - Updated several bios and notebook
- 29/06/11 - Several meetings placed onto notebook
- 28/06/11 - Many small layout changes made
- 27/06/11 - Several meetings placed onto notebook
- 24/06/11 - Added notebook calendar
- 23/06/11 - Fixed sponsor template
- 21/06/11 - Remaining protocols added
- 20/06/11 - First protocols added
- 17/06/11 - Photos added at lab environment
- 15/06/11 - Created footer
- 15/06/11 - Created all pages
- 12/06/11 - Created first version of header
- 02/06/11 - Temporary wiki online
