"
Team:Brown-Stanford/Lab/Protocols/DM3 plates
From 2011.igem.org
-
REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
-
Lab
-
Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
DM3 Plates
DM3 plates:
- Agar 200ml 4%
- Sodium Succinate 500ml 1M pH 7.3
- Casamino Acids 100ml 5%
- Yeast Extract 50ml 10%
- K2HPO4 3.5% + KH2PO4 1.5% in 100ml
- Glucose 15ml 20%
- MgCl2 20ml 1M
- BSA 5ml 2%
Notes
- Autoclave everything except BSA
- Filter sterilize BSA and add when mixture is ~55˚C
