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Team:Brown-Stanford/Lab/Protocols/CementationColumn
From 2011.igem.org
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REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
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Jamboree
Biocementation Column
Materials
- 15 mL falcon tube
- 20 g of Lunar/Martian regolith per tube
- A peristaltic pump
- Two feet of rubber tubing, epoxy glue, fabric matting
- Heat Lamp
- 30 mL of S. pasteurii culture (OD600 1.2)
Procedure
- The columns were first prewet with 20 mL of sterile Bang media.
- The bacteria culture was draw into the feed tube, and allowed to settle in the column overnight
- Cementation solution (1.5 M equimolar urea and calcium chloride) was fed into the feed tube, which was then placed into a constant loop with the output tube
- The column was heated for 5 days, and allowed to cycle.
