Protocol Code: M1

BG-11

(Taken from Meeks Lab at University of California, Davis)

Stock solutions for BG-11

Stock 1

• 0.1g/liter Na₂MG EDTA
• 0.6g/liter Ferric ammonium citrate
• 0.6g/liter Citric acid•1H₂O
• 3.6g/liter CaCl₂•2H₂O

Filter sterilize into a sterile bottle or autoclave.

Stock 2

• 7.5g/liter MgSO₄•7H₂O

Filter sterilize into a sterile bottle or autoclave.

Stock 3

• 4.0g/liter K₂HPO₄•3H₂O or 3.05g/liter K₂HPO₄

Filter sterilize into a sterile bottle or autoclave.

Stock 5 (Microelements)

Stock 5 (Microelements):

• 2.86g/liter H₃BO₃
• 1.81g/liter MnCl₂•4H₂O
• 0.222g/liter ZnSO₄•7H₂O
• 0.079g/liter CuSO₄•5H₂O
• 0.050g/liter COCl₂•6H₂O
• 0.391g/liter NaMoO₄•2H₂O or 0.018g/liter MoO₄ (85%)

Basic BG11 medium

Combine the following stock solutions (for 1 liter):

• 10 mL Stock 1
• 10 mL Stock 2
• 10 mL Stock 3
• 0.02 g Na₂CO₃
• 1.0 mL Stock 5
• 1.5 g NaNO₃

Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 mL/125 mL flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO₃.