Team:Brown-Stanford/Lab/Protocols/BG11

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BG-11

(Taken from [http://microbiology.ucdavis.edu/meeks/BG11medium.html|UC Davis])

Stock solutions for BG-11

Stock 1: 0.1g/liter Na2MG EDTA 0.6g/liter Ferric ammonium citrate Citric acid*1H2O 0.6g/liter CaCl2 . 2H2O 3.6g/liter Filter sterilize into a sterile bottle or autoclave Stock 2: MgSO4 . 7H2O 7.5g/liter Filter sterilize into a sterile bottle or autoclave Stock 3: K2HPO4 . 3H2O 4.0g/liter or K2HPO4 3.05g/liter Filter sterilize into a sterile bottle or autoclave Stock 5 (Microelements): H3BO3 2.86g/liter MnCl2 . 4H2O 1.81g/liter ZnSO4 . 7H2O 0.222g/liter CuSO4 . 5H2O 0.079g/liter COCl2 . 6H2O 0.050g/liter NaMoO4 . 2H2O 0.391g/liter or MoO4 (85%) 0.018g/liter

B) For basic BG11 medium combine the following stock solutions: Stock Solution Per Liter of medium Stock 1 10 ml Stock 2 10 ml Stock 3 10 ml Na2CO3 0.02g Stock 5 1.0 ml NaNO3 1.5g

Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11o don't add NaNO3.