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User contributions
From 2011.igem.org
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- 14:51, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:48, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/algaeglassbeads (Created page with "{{Banner}} 1. Transfer 50ml of algae culture into four 50ml screw top tubes. 2. Place all four tubes in the eppendorf centrifuge at 800rpm for 9 minutes at 20-22°C. 3. Immedi...") (top)
- 14:48, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:46, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/pegtransformation (Created page with "{{Banner}} <p style="color:#000000">1. Add 9ml of 8% mannitol to a petri dish.</p> <p style="color:#000000">2. Using a spatula, put 7 day old moss from 2-3 PPNH4 plates in the p...")
- 14:45, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:44, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/pcr (Created page with "{{Banner}} <p style="color:#000000">1. Program desired cycle into PCR machine. Cycle used was as follows:</p> <p style="color:#000000">94°C - Initial Den...") (top)
- 14:44, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:42, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/electrophoresis (Created page with "{{Banner}} <p style="color:#000000">1. Measure 0.6g of agarose and add to 60 mL TAE Buffer</p> <p style="color:#000000">2. Mix throroughly</p> <p style="color:#000000">3. Heat u...") (top)
- 14:42, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:41, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/miniprep (Created page with "{{Banner}} <p style="color:#000000">1. Resuspend pelleted bacterial cells in 250 µl Buffer P1 and transfer to a microcentrifuge tube. </p> <p style="color:#000000">2. Add 250 ...")
- 14:40, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:40, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:39, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/glycerolstock (Created page with "{{Banner}} <p style="color:#000000">1. Take 500µL of a previously prepared culture and place into a screw-top eppendorf tube</p> <p style="color:#000000">2. Add 500µL of Glyce...") (top)
- 14:38, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:37, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/cultureprep (Created page with "{{Banner}} <p style="color:#000000">1. Select a colony by sampling it with a wooden pick</p> <p style="color:#000000">2. Place into the appropriate antibiotic LB broth</p> <p st...") (top)
- 14:36, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:36, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 14:35, 21 September 2011 (diff | hist) N Team:UEA-JIC Norwich/transformation (Created page with "{{Banner}} <p style="color:#000000">1. Thaw a tube of NEB 5-alpha Competent E.coli cells on ice for 10 minutes</p> <p style="color:#000000">2. Mark the location of the Biobrick ...")
- 14:34, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Methods
- 13:50, 21 September 2011 (diff | hist) Template:Boxes
- 13:47, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Our advisors (top)
- 11:30, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Project
- 11:23, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Project
- 10:48, 21 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices/Interviewten
- 10:02, 21 September 2011 (diff | hist) Template:Banner (top)
- 09:59, 21 September 2011 (diff | hist) Template:Banner (Undo revision 147350 by Benjevans1 (talk))
- 09:58, 21 September 2011 (diff | hist) Template:Banner (Undo revision 147532 by Benjevans1 (talk))
- 21:43, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Attributions
- 21:42, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Attributions
- 20:49, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Nittygritty-algae
- 19:26, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Human practices
- 19:24, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weekfive
- 19:23, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weekfive
- 19:17, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Project
- 18:00, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Project
- 17:55, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Project
- 17:39, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weekfifteen (Created page with "{{Banner}} __NOTOC__ <br>Monday 19th September 2011 <br> Alistair, Abbie, Mark and Mario worked on the wiki with advice from Richard Kelwick on layout. <br>Tuesday 20th Septembe...")
- 17:34, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weekfourteen (Created page with "{{Banner}} __NOTOC__ <br>Monday 12th September <br> These were then ran on a gel which gave a mixture of good and bad results yet again. A restriction digest was performed on Ali...")
- 17:33, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weekthirteen (Created page with "{{Banner}} __NOTOC__ <br>Monday 4th September <br> Ben Jevans carried out a PCR of the miniprepped Ble and G-Luc plasmids and ran a gel, then performed another mini-prep and gel ...")
- 17:32, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weektwelve (Created page with "{{Banner}} __NOTOC__ <br>Monday 29th August <br> Alistair performed PCR upon the CaMV promoter and ran on a gel which gave positive results showing a sequence on gels of about th...")
- 17:30, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weekeleven (Created page with "{{Banner}} __NOTOC__ <br>Monday 22nd August <br> A nanodrop was carried out by Ben Jevans in the morning on the original miniprepped PCR G-Luc products from last week, results lo...")
- 17:28, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weekten
- 17:27, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weekten (Created page with "{{Banner}} __NOTOC__ <br>Monday 15th August <br> The Moss party consisting of Alistair, Abbie and Ben Hardy commence the day by transferring grown protoplasts to new plates; prot...")
- 17:25, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weeknine (Created page with "{{Banner}} __NOTOC__ <br>Monday 8th August 2011 <br> Abbie, Ben Hardy and Alistair did another PCR on the CaMV promoter due to it being unsuccessful the previous day and then ran...")
- 17:24, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weekeight
- 17:24, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weekeight
- 17:23, 20 September 2011 (diff | hist) N Team:UEA-JIC Norwich/Weekeight (Created page with "{{Banner}} __NOTOC__ <br>Monday 1st August 2011 <br> Team meeting discussing finance and outreach. Alistair and Ben Hardy grew up bacteria containing a rbs biobrick. Ben Jevans a...")
- 17:21, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weekseven
- 17:20, 20 September 2011 (diff | hist) Team:UEA-JIC Norwich/Weeksix
- 17:17, 20 September 2011 (diff | hist) N Talk:Team:UEA-JIC Norwich/Weekfive (Created page with "{{Banner}} <br>Monday 11th July 2011 <br> A start to the week with everyone contemplating what has already been done and what we think is best to move forward successfully with ...") (top)
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