Team:Fatih Turkey/Experiments
From 2011.igem.org
CHARACTERIZATION Before starting assays; we needed to be sure that our genes had been cloned correctly. In order to use our parts in our experiments, we confirmed our parts via electrophoresis as it can be seen in image A. Following parts are fully confirmed:
After electrophoresis results; we furthermore performed sequencing and imaging tests to characterize our BioBricks. For BBa_541505 and BBa_541506; we added a GFP sequence and transformated into E.coli. This has characterized our main proteins. cDNA sequencing for these parts was also done successfully. |