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Team:Brown-Stanford/Lab/Protocols/Bang
From 2011.igem.org
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REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
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Lab
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Partners
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Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocol Code: M7
Bang Media
By Percentage
- 1% tryptone
- 0.5% yeast extract
- 0.45 tricine
- 0.5% (NH4)2SO4
- 0.2% glutamic acid
- 1% urea
By Mass per Liter
- 10g tryptone
- 5g yeast extract
- 4.5g tricine
- 5g (NH4)2SO4
- 2g glutamic acid
- 10g urea
- Optional: 15g agar (for plates)
Notes
- Mix in 200mL of water (without agar) to make a 5x solution.
- Needs to be at a pH of 8.5
