Team:Brown-Stanford/Lab/Protocols/BG11

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BG-11

(Taken from [http://microbiology.ucdavis.edu/meeks/BG11medium.html| UC Davis])

Stock solutions for BG-11

Stock 1: 0.1g/liter Na₂MG EDTA 0.6g/liter Ferric ammonium citrate Citric acid•1H₂O 0.6g/liter CaCl₂•2H₂O 3.6g/liter Filter sterilize into a sterile bottle or autoclave Stock 2: MgSO₄•7H₂O 7.5g/liter Filter sterilize into a sterile bottle or autoclave Stock 3: K_2HPO4•3H₂O 4.0g/liter or K₂HPO₄ 3.05g/liter Filter sterilize into a sterile bottle or autoclave Stock 5 (Microelements): H3BO3 2.86g/liter MnCl₂•4H₂O 1.81g/liter ZnSO₄•7H₂O 0.222g/liter CuSO₄•5H₂O 0.079g/liter COCl₂•6H₂O 0.050g/liter NaMoO₄•2H₂O 0.391g/liter or MoO₄ (85%) 0.018g/liter

B) For basic BG11 medium combine the following stock solutions: Stock Solution Per Liter of medium

10 mL Stock 1
10 mL Stock 2
10 mL Stock 3
0.02 g Na₂CO₃
1.0 mL Stock 5
1.5 g NaNO₃

Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO₃.