"
Team:Brown-Stanford/Lab/Protocols/LB
From 2011.igem.org
-
REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
-
Lab
-
Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
LB (Lysogeny broth)
- Add 250 mL of dH2O to a graduated cyclindar.
|
|
- Mix powder well to bring into solution
- Add dH2O to total volume of 500 mL and transfer to 1 L flask
- Put on stirring hot plate and heat to boil for 1 min while stirring.
- Transfer to 1 L pyrex jar and label with autoclave tape.
- Autoclave at liquid setting for 20 minutes in a basin making sure to loosen top
- Let agar cool to ~55C (you should be able to pick up the jar without a glove)
