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Team:Brown-Stanford/Lab/Protocols/DM3 plates
From 2011.igem.org
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REGObricks
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PowerCell
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FRETcetera
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Outreach
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Jamboree
DM3 Plates
DM3 plates:
- Agar 200ml 4%
- Sodium Succinate 500ml 1M pH 7.3
- Casamino Acids 100ml 5%
- Yeast Extract 50ml 10%
- K2HPO4 3.5% + KH2PO4 1.5% in 100ml
- Glucose 15ml 20%
- MgCl2 20ml 1M
- BSA 5ml 2%
Notes
- Filter sterilize BSA and add when mixture is ~55˚C
