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Contents 1 Protocols 1.1 Preparations 1.2 Cloning 1.3 BioBrick characterization

Protocols

Preparations

• Preparing media
• Preparation of chemically competent cells
• Preparation of electrocompetent cells

Cloning

• Miniprepping
• Digestion
• Ligations
• Transforming chemically competent cells
• Transforming electrocompetent cells
• Colony PCR

BioBrick characterization

• Growth curve measurement
• Freeze/thaw survival measurement