Team:Amsterdam/11 July 2011
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Revision as of 12:59, 19 July 2011
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11-07-2011 | Meeting 18
Workplan
- A platereader is available
- Only meassurments can be done at RT and 37 degrees Celcius
- No difference can be seen between promoter and protein folding at cold temperatures
- Maybe with qPCR?
- We need to look into this, it is expensive, will it work at low temperatures?
- Maybe with qPCR?
- Can we use GFP at cold temperatures for promoter characterisation?
- Search for other useful proteins like GFP
- Synthesized genes are ordered
Labwork
- Ensembly of 2 plasmids will be done
- First miniprep, then test on gel
- Make glycerol stock of the good colonies
- First miniprep, then test on gel
Funding
- Kickstarter had some delay, but will be online next week
- VU/UVA funding is still in progress
- Isogen sponsored us a photospectrometer and a PCR, to borrow it for some time
Modelling
- How to model the promoter activity
- Meassure OD and fluorescence
Human Outreach/PR
- EHBU isn't useful for us
- We won't be there
- We will concentrate on Nemo/Naturalis
- Ad valvas, we can have a short story in September
Labwork
- Plates with transformants were counted
- Multiple colonies were grown ON 37 degrees celsius in liquid culture