Team:Amsterdam/6 July 2011
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''Brainstorm session with Douwe Molenaar and Isoude Kuijper'' | ''Brainstorm session with Douwe Molenaar and Isoude Kuijper'' |
Revision as of 12:52, 19 July 2011
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06-07-2011
Ideas for modelling
Brainstorm session with Douwe Molenaar and Isoude Kuijper
Steady state model of promoters
- Information needed for model
- Stability
- Transcription
- Measuring with qPCR
- Translation
- Fluorescence
- Transcription
- Growth rate
- Stability
- Information needed for all promoters
- Inducible promoter
- IPTG promoter is also dependable on a lactose receptor/transporter
- Cold shock promoter
- Inducible promoter
- Temperature dependent
- The inducible promoters are also concentration dependent
- Need to look into literature for already available information
- The inducible promoters are also concentration dependent
The cold chock promoter
- How does this promoter work?
Cryobrick
- What concentration is needed?
- How many targets do the chaperone proteins have?
Enzyme activity
- Enzyme activity is also temperature dependent
Lab Work
- Made a lot of plates and prepared Bba_R0010 and BBa_B0034 for miniprep testing tomorrow