Team:Amsterdam/6 July 2011

From 2011.igem.org

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=Ideas for modelling=
=Ideas for modelling=
''Brainstorm session with Douwe Molenaar and Isoude Kuijper''  
''Brainstorm session with Douwe Molenaar and Isoude Kuijper''  
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==Enzyme activity==  
==Enzyme activity==  
* Enzyme activity is also temperature dependent
* Enzyme activity is also temperature dependent
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Revision as of 12:09, 6 July 2011

Contents

Ideas for modelling

Brainstorm session with Douwe Molenaar and Isoude Kuijper

Steady state model of promoters

  • Information needed for model
    • Stability
      • Transcription
        • Measuring with qPCR
      • Translation
        • Fluorescence
    • Growth rate
  • Information needed for all promoters
    • Inducible promoter
      • IPTG promoter is also dependable on a lactose receptor/transporter
    • Cold shock promoter
  • Temperature dependent
    • The inducible promoters are also concentration dependent
      • Need to look into literature for already available information

The cold chock promoter

  • How does this promoter work?

Cryobrick

  • What concentration is needed?
    • How many targets do the chaperone proteins have?

Enzyme activity

  • Enzyme activity is also temperature dependent

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