Team:British Columbia/Model3

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Team: British Columbia - 2011.igem.org

Monoterpene Synthase Structural Modeling

Objective: To identify mutations that may enhance the efficiency of terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.

Model Creators: Joe Ho, Samuel Wu

Model Advisors: Shing Hei Zhan, Alina Chan

Three-dimensional structure of the 3 terpene synthases

File:UBCiGEM superimposed synthases.jpeg

Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified an experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.

Reactive site of the three synthases

References

1. N. Eswar, M. A. Marti-Renom, B. Webb, M. S. Madhusudhan, D. Eramian, M. Shen, U. Pieper, A. Sali. Comparative Protein Structure Modeling With MODELLER. Current Protocols in Bioinformatics, John Wiley & Sons, Inc., Supplement 15, 5.6.1-5.6.30, 2006.

@@ Line 11: / Line 11: @@
 <html><h3>Three-dimensional structure of the 3 terpene synthases</h3></html><p></p>
 [[File:UBCiGEM superimposed synthases.jpeg]]
-<p></p>Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified the experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.<p></p>
+<p></p>Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified an experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.<p></p>
 <b>Reactive site of the three synthases</b><p></p>
 [[File:UBCiGEM model3 reactive site.jpg]]
+<html><h3>References</h3></html><p></p>
+. N. Eswar, M. A. Marti-Renom, B. Webb, M. S. Madhusudhan, D. Eramian, M. Shen, U. Pieper, A. Sali. Comparative Protein Structure Modeling With MODELLER. Current Protocols in Bioinformatics, John Wiley & Sons, Inc., Supplement 15, 5.6.1-5.6.30, 2006.