Team:British Columbia/Model3

From 2011.igem.org

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</style><div id="bod"><center><h3>Monoterpene Synthase Structural Modeling</h3></center></html>
</style><div id="bod"><center><h3>Monoterpene Synthase Structural Modeling</h3></center></html>
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<b>Objective:</b><p></p>
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<b>Objective: To identify mutations that may enhance the efficiency of terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.</b><p></p>
<b>Model Creators:</b> Joe Ho, Samuel Wu <p></p>
<b>Model Creators:</b> Joe Ho, Samuel Wu <p></p>
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<b>Model Advisors:</b> Shing Hei Zhan, Alina Chan <p></p>
<b>Model Advisors:</b> Shing Hei Zhan, Alina Chan <p></p>
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<html><h3>in silico Mutant Screening</h3></html>
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<html><h3>Three-dimensional structure of the 3 terpene synthases</h3></html><p></p>
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[[File:UBCiGEM superimposed synthases.jpeg]]
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<html><h3>Materials and Methods</h3></html>
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<p></p>Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified the experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.<p></p>
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<html><h3>Results</h3></html>
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<b>Three-dimensional structure of the terpene synthases</b><p></p>
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[[File:UBCiGEM superimposed synthases.tif]]
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<b>Reactive site of the three synthases</b><p></p>
<b>Reactive site of the three synthases</b><p></p>
[[File:UBCiGEM model3 reactive site.jpg]]
[[File:UBCiGEM model3 reactive site.jpg]]
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<b>Change in substrate binding affinity</b><p></p>
 

Revision as of 01:46, 29 October 2011

Team: British Columbia - 2011.igem.org

Monoterpene Synthase Structural Modeling

Objective: To identify mutations that may enhance the efficiency of terpene synthesis, we constructed three-dimensional models of the terpene synthase proteins.

Model Creators: Joe Ho, Samuel Wu

Model Advisors: Shing Hei Zhan, Alina Chan

Three-dimensional structure of the 3 terpene synthases

File:UBCiGEM superimposed synthases.jpeg

Superimposition of the 3D structures of alpha-pinene synthase (blue), beta-pinene synthase (red), and limonene synthase (green). We used MODELLER (1) to automate homology-based 3D structure prediction. We identified the experimentally determined 3D structure of a taxadiene synthase from Pacific Yew (PDB ID: 3P5R) as an appropriate template.

Reactive site of the three synthases

UBCiGEM model3 reactive site.jpg

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