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Team:Brown-Stanford/Lab/Protocols/SOB
From 2011.igem.org
(Difference between revisions)
(Created page with "{{:Team:Brown-Stanford/Templates/Main}} Per liter of SOB: *5 g yeast extract *20 g tryptone *10 mL NaCl *2.5 mL KCl *20 mL MgSO4 {{:Team:Brown-Stanford/Templates/Foot}}") |
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{{:Team:Brown-Stanford/Templates/Main}} | {{:Team:Brown-Stanford/Templates/Main}} | ||
| + | |||
| + | == SOB == | ||
| + | |||
| + | Composition: | ||
| + | *0.5% (w/v) yeast extract | ||
| + | *2% (w/v) tryptone | ||
| + | *10 mM NaCl | ||
| + | *2.5 mM KCl | ||
| + | *20 mM MgSO4 | ||
Per liter of SOB: | Per liter of SOB: | ||
*5 g yeast extract | *5 g yeast extract | ||
*20 g tryptone | *20 g tryptone | ||
| - | *10 mL NaCl | + | *10 mL NaCl (1M stock) |
| - | *2.5 mL KCl | + | *2.5 mL KCl (1M stock) |
| - | *20 mL MgSO4 | + | *20 mL MgSO4 (1M stock) |
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Revision as of 21:31, 22 June 2011
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SOB
Composition:
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter of SOB:
- 5 g yeast extract
- 20 g tryptone
- 10 mL NaCl (1M stock)
- 2.5 mL KCl (1M stock)
- 20 mL MgSO4 (1M stock)
