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Team:Amsterdam/Notebook/Protocols/Transforming electrocompetent Cells
From 2011.igem.org
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| - | + | *Prewarmed [https://2011.igem.org/Team:Amsterdam/Notebook/Protocols/Preparing_Media SOC] | |
| + | *[https://2011.igem.org/Team:Amsterdam/Notebook/Protocols/Making_electrocompetent_Cells Electrocompetent cells] | ||
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===Procedure=== | ===Procedure=== | ||
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Revision as of 10:09, 21 September 2011
Contents |
Transforming electrocompetent cells
Overview
We use a protocol commonly used in our host lab provided by Lisette Anink. The main advantage of electrocompetent cells compared to chemically competent cells is a 1-2 orders of magnitude higher level of competence. The disadvantage, though, is the high price of electroporation cuvettes and the implications this has on maximum number of transformations that can be performed in a single experiment.
Materials
- Prewarmed SOC
- Electrocompetent cells
Procedure
