Team:Brown-Stanford/Lab/Protocols/LB

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Revision as of 22:50, 11 June 2011

Brown-Stanford
iGEM

LB (Lysogeny broth)

  1. Add 250 mL of dH2O to a graduated cyclindar.
    1. Weigh out 20g of premix LB Agar powder (VWR DF0445-17)
    1. 5.0 g tryptone
    2. 2.5 g yeast extract
    3. 5.0 g NaCl
    4. 7.5 g agar
  1. Mix powder well to bring into solution
  2. Add dH2O to total volume of 500 mL and transfer to 1 L flask
  3. Put on stirring hot plate and heat to boil for 1 min while stirring.
  4. Transfer to 1 L pyrex jar and label with autoclave tape.
  5. Autoclave at liquid setting for 20 minutes in a basin making sure to loosen top
  6. Let agar cool to ~55C (you should be able to pick up the jar without a glove)

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