April
|
M | T | W | T | F | S | S
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|
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| [http://2011.igem.org/Team:Amsterdam/1_April_2011 1]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_April_2011&action=edit 2]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_April_2011&action=edit 3]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_April_2011&action=edit 4]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_April_2011&action=edit 5]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_April_2011&action=edit 6]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_April_2011&action=edit 7]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_April_2011&action=edit 8]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_April_2011&action=edit 9]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_April_2011&action=edit 10]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_April_2011&action=edit 11]
| [http://2011.igem.org/Team:Amsterdam/12_April_2011 12]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_April_2011&action=edit 13]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_April_2011&action=edit 14]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_April_2011&action=edit 15]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_April_2011&action=edit 16]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_April_2011&action=edit 17]
|
[http://2011.igem.org/Team:Amsterdam/18_April_2011 18]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_April_2011&action=edit 19]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_April_2011&action=edit 20]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_April_2011&action=edit 21]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_April_2011&action=edit 22]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_April_2011&action=edit 23]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_April_2011&action=edit 24]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_April_2011&action=edit 25]
| [http://2011.igem.org/Team:Amsterdam/26_April_2011 26]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_April_2011&action=edit 27]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_April_2011&action=edit 28]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_April_2011&action=edit 29]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_April_2011&action=edit 30]
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May
|
M | T | W | T | F | S | S
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|
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|
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_May_2011&action=edit 1]
|
[http://2011.igem.org/Team:Amsterdam/2_May_2011 2]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_May_2011&action=edit 3]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_May_2011&action=edit 4]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_May_2011&action=edit 5]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_May_2011&action=edit 6]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_May_2011&action=edit 7]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_May_2011&action=edit 8]
|
[http://2011.igem.org/Team:Amsterdam/9_May_2011 9]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_May_2011&action=edit 10]
| [http://2011.igem.org/Team:Amsterdam/11_May_2011 11]
| [http://2011.igem.org/Team:Amsterdam/12_May_2011 12]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_May_2011&action=edit 13]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_May_2011&action=edit 14]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_May_2011&action=edit 15]
|
[http://2011.igem.org/Team:Amsterdam/16_May_2011 16]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_May_2011&action=edit 17]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_May_2011&action=edit 18]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_May_2011&action=edit 19]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_May_2011&action=edit 20]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_May_2011&action=edit 21]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_May_2011&action=edit 22]
|
[http://2011.igem.org/Team:Amsterdam/23_May_2011 23]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_May_2011&action=edit 24]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_May_2011&action=edit 25]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_May_2011&action=edit 26]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_May_2011&action=edit 27]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_May_2011&action=edit 28]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_May_2011&action=edit 29]
|
[http://2011.igem.org/Team:Amsterdam/30_May_2011 30]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_May_2011&action=edit 31]
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June
|
M | T | W | T | F | S | S
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|
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_June_2011&action=edit 1]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_June_2011&action=edit 2]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_June_2011&action=edit 3]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_June_2011&action=edit 4]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_June_2011&action=edit 5]
|
[http://2011.igem.org/Team:Amsterdam/6_June_2011 6]
| [http://2011.igem.org/Team:Amsterdam/7_June_2011 7]
| [http://2011.igem.org/Team:Amsterdam/8_June_2011 8]
| [http://2011.igem.org/Team:Amsterdam/9_June_2011 9]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_June_2011&action=edit 10]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_June_2011&action=edit 11]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/12_June_2011&action=edit 12]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_June_2011&action=edit 13]
| [http://2011.igem.org/Team:Amsterdam/14_June_2011 14]
| [http://2011.igem.org/Team:Amsterdam/15_June_2011 15]
| [http://2011.igem.org/Team:Amsterdam/16_June_2011 16]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_June_2011&action=edit 17]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_June_2011&action=edit 18]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_June_2011&action=edit 19]
|
[http://2011.igem.org/Team:Amsterdam/20_June_2011 20]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_June_2011&action=edit 21]
| [http://2011.igem.org/Team:Amsterdam/22_June_2011 22]
| [http://2011.igem.org/Team:Amsterdam/23_June_2011 23]
| [http://2011.igem.org/Team:Amsterdam/24_June_2011 24]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_June_2011&action=edit 25]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_June_2011&action=edit 26]
|
[http://2011.igem.org/Team:Amsterdam/27_June_2011 27]
| [http://2011.igem.org/Team:Amsterdam/28_June_2011 28]
| [http://2011.igem.org/Team:Amsterdam/29_June_2011 29]
| [http://2011.igem.org/Team:Amsterdam/30_June_2011 30]
|
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July
|
M | T | W | T | F | S | S
|
|
|
|
| [http://2011.igem.org/Team:Amsterdam/1_July_2011 1]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_July_2011&action=edit 2]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_July_2011&action=edit 3]
|
[http://2011.igem.org/Team:Amsterdam/4_July_2011 4]
| [http://2011.igem.org/Team:Amsterdam/5_July_2011 5]
| [http://2011.igem.org/Team:Amsterdam/6_July_2011 6]
| [http://2011.igem.org/Team:Amsterdam/7_July_2011 7]
| [http://2011.igem.org/Team:Amsterdam/8_July_2011 8]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_July_2011&action=edit 9]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_July_2011&action=edit 10]
|
[http://2011.igem.org/Team:Amsterdam/11_July_2011 11]
| [http://2011.igem.org/Team:Amsterdam/12_July_2011 12]
| [http://2011.igem.org/Team:Amsterdam/13_July_2011 13]
| [http://2011.igem.org/Team:Amsterdam/14_July_2011 14]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_July_2011&action=edit 15]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_July_2011&action=edit 16]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_July_2011&action=edit 17]
|
[http://2011.igem.org/Team:Amsterdam/18_July_2011 18]
| [http://2011.igem.org/Team:Amsterdam/19_July_2011 19]
| [http://2011.igem.org/Team:Amsterdam/20_July_2011 20]
| [http://2011.igem.org/Team:Amsterdam/21_July_2011 21]
| [http://2011.igem.org/Team:Amsterdam/22_July_2011 22]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_July_2011&action=edit 23]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_July_2011&action=edit 24]
|
[http://2011.igem.org/Team:Amsterdam/25_July_2011 25]
| [http://2011.igem.org/Team:Amsterdam/26_July_2011 26]
| [http://2011.igem.org/Team:Amsterdam/27_July_2011 27]
| [http://2011.igem.org/Team:Amsterdam/28_July_2011 28]
| [http://2011.igem.org/Team:Amsterdam/29_July_2011 29]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_July_2011&action=edit 30]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_July_2011&action=edit 31]
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August
|
M | T | W | T | F | S | S
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_August_2011&action=edit 1]
| [http://2011.igem.org/Team:Amsterdam/2_August_2011 2]
| [http://2011.igem.org/Team:Amsterdam/3_August_2011 3]
| [http://2011.igem.org/Team:Amsterdam/4_August_2011 4]
| [http://2011.igem.org/Team:Amsterdam/5_August_2011 5]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_August_2011&action=edit 6]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_August_2011&action=edit 7]
|
[http://2011.igem.org/Team:Amsterdam/8_August_2011 8]
| [http://2011.igem.org/Team:Amsterdam/9_August_2011 9]
| [http://2011.igem.org/Team:Amsterdam/10_August_2011 10]
| [http://2011.igem.org/Team:Amsterdam/11_August_2011 11]
| [http://2011.igem.org/Team:Amsterdam/12_August_2011 12]
| [http://2011.igem.org/Team:Amsterdam/13_August_2011 13]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_August_2011&action=edit 14]
|
[http://2011.igem.org/Team:Amsterdam/15_August_2011 15]
| [http://2011.igem.org/Team:Amsterdam/16_August_2011 16]
| [http://2011.igem.org/Team:Amsterdam/17_August_2011 17]
| [http://2011.igem.org/Team:Amsterdam/18_August_2011 18]
| [http://2011.igem.org/Team:Amsterdam/19_August_2011 19]
| [http://2011.igem.org/Team:Amsterdam/20_August_2011 20]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_August_2011&action=edit 21]
|
[http://2011.igem.org/Team:Amsterdam/22_August_2011 22]
| [http://2011.igem.org/Team:Amsterdam/23_August_2011 23]
| [http://2011.igem.org/Team:Amsterdam/24_August_2011 24]
| [http://2011.igem.org/Team:Amsterdam/25_August_2011 25]
| [http://2011.igem.org/Team:Amsterdam/26_August_2011 26]
| [http://2011.igem.org/Team:Amsterdam/27_August_2011 27]
| [http://2011.igem.org/Team:Amsterdam/28_August_2011 28]
|
[http://2011.igem.org/Team:Amsterdam/29_August_2011 29]
| [http://2011.igem.org/Team:Amsterdam/30_August_2011 30]
| [http://2011.igem.org/Team:Amsterdam/31_August_2011 31]
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|
September
|
M | T | W | T | F | S | S
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|
|
| [http://2011.igem.org/Team:Amsterdam/1_September_2011 1]
| [http://2011.igem.org/Team:Amsterdam/2_September_2011 2]
| [http://2011.igem.org/Team:Amsterdam/3_September_2011 3]
| [http://2011.igem.org/Team:Amsterdam/4_September_2011 4]
|
[http://2011.igem.org/Team:Amsterdam/5_September_2011 5]
| [http://2011.igem.org/Team:Amsterdam/6_September_2011 6]
| [http://2011.igem.org/Team:Amsterdam/7_September_2011 7]
| [http://2011.igem.org/Team:Amsterdam/8_September_2011 8]
| [http://2011.igem.org/Team:Amsterdam/9_September_2011 9]
| [http://2011.igem.org/Team:Amsterdam/10_September_2011 10]
| [http://2011.igem.org/Team:Amsterdam/11_September_2011 11]
|
[http://2011.igem.org/Team:Amsterdam/12_September_2011 12]
| [http://2011.igem.org/Team:Amsterdam/13_September_2011 13]
| [http://2011.igem.org/Team:Amsterdam/14_September_2011 14]
| [http://2011.igem.org/Team:Amsterdam/15_September_2011 15]
| [http://2011.igem.org/Team:Amsterdam/16_September_2011 16]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_September_2011&action=edit 17]
| [http://2011.igem.org/Team:Amsterdam/18_September_2011 18]
|
[http://2011.igem.org/Team:Amsterdam/19_September_2011 19]
| [http://2011.igem.org/Team:Amsterdam/20_September_2011 20]
| [http://2011.igem.org/Team:Amsterdam/21_September_2011 21]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_September_2011&action=edit 22]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_September_2011&action=edit 23]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_September_2011&action=edit 24]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_September_2011&action=edit 25]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_September_2011&action=edit 26]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_September_2011&action=edit 27]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_September_2011&action=edit 28]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_September_2011&action=edit 29]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_September_2011&action=edit 30]
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October
|
M | T | W | T | F | S | S
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|
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|
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_October_2011&action=edit 1]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_October_2011&action=edit 2]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_October_2011&action=edit 3]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_October_2011&action=edit 4]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_October_2011&action=edit 5]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_October_2011&action=edit 6]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_October_2011&action=edit 7]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_October_2011&action=edit 8]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_October_2011&action=edit 9]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_October_2011&action=edit 10]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_October_2011&action=edit 11]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/12_October_2011&action=edit 12]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_October_2011&action=edit 13]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_October_2011&action=edit 14]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_October_2011&action=edit 15]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_October_2011&action=edit 16]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_October_2011&action=edit 17]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_October_2011&action=edit 18]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_October_2011&action=edit 19]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_October_2011&action=edit 20]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_October_2011&action=edit 21]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_October_2011&action=edit 22]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_October_2011&action=edit 23]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_October_2011&action=edit 24]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_October_2011&action=edit 25]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_October_2011&action=edit 26]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_October_2011&action=edit 27]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_October_2011&action=edit 28]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_October_2011&action=edit 29]
| [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_October_2011&action=edit 30]
|
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_October_2011&action=edit 31]
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Transformation check
Only the positive control showed colonies on the plate
- The construct making will be redone
Constructs
Construct were made according to assembly protocol of Delft 2010.
Digestion
Digestion of vectors with SpeI and PstI:
- R0010_pSB1A2
- K206000_pSBA3
- R0010_B0034_pSB1A2
Digestion of inserts with XbaI and PstI:
- Shednak
- CspC
- Cpn10
- Cpn60
- B0034_Cpn10_pSB1A2
- B0034_Cpn60_pSB1A2
- B0034_CspC_pSB1A2
Purification
The vectors were purified with size selective purification. The inserts were purified on agarose gel.
Ligation
The following constructs were ligated ON at 16 degrees Celcius:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn10
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn10
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
Growth curves
Still measuring the growth curves for 4 and 8°C