Team:Amsterdam/6 July 2011

From 2011.igem.org

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{{:Team:Amsterdam/Header}}
{{:Team:Amsterdam/Header}}
{{:Team:Amsterdam/Notebook/Calendar}}
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= 06-06-2011 =
=Ideas for modelling=
=Ideas for modelling=
''Brainstorm session with Douwe Molenaar and Isoude Kuijper''  
''Brainstorm session with Douwe Molenaar and Isoude Kuijper''  
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==Enzyme activity==  
==Enzyme activity==  
* Enzyme activity is also temperature dependent
* Enzyme activity is also temperature dependent
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=Lab Work=
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*Made a lot of plates and prepared Bba_R0010 and BBa_B0034 for miniprep testing tomorrow
{{:Team:Amsterdam/Footer}}
{{:Team:Amsterdam/Footer}}

Revision as of 11:39, 7 July 2011

April
MTWTFSS
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[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_April_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_April_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_April_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_April_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_April_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_April_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_April_2011&action=edit 10]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_April_2011&action=edit 11] [http://2011.igem.org/Team:Amsterdam/12_April_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_April_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_April_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_April_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_April_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_April_2011&action=edit 17]
[http://2011.igem.org/Team:Amsterdam/18_April_2011 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_April_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_April_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_April_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_April_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_April_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_April_2011&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_April_2011&action=edit 25] [http://2011.igem.org/Team:Amsterdam/26_April_2011 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_April_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_April_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_April_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_April_2011&action=edit 30]
May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_May_2011&action=edit 1]
[http://2011.igem.org/Team:Amsterdam/2_May_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_May_2011&action=edit 8]
[http://2011.igem.org/Team:Amsterdam/9_May_2011 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_May_2011&action=edit 10] [http://2011.igem.org/Team:Amsterdam/11_May_2011 11] [http://2011.igem.org/Team:Amsterdam/12_May_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_May_2011&action=edit 15]
[http://2011.igem.org/Team:Amsterdam/16_May_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_May_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_May_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_May_2011&action=edit 22]
[http://2011.igem.org/Team:Amsterdam/23_May_2011 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_May_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_May_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_May_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_May_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_May_2011&action=edit 29]
[http://2011.igem.org/Team:Amsterdam/30_May_2011 30] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_May_2011&action=edit 31]
June
MTWTFSS
    [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_June_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_June_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_June_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_June_2011&action=edit 5]
[http://2011.igem.org/Team:Amsterdam/6_June_2011 6] [http://2011.igem.org/Team:Amsterdam/7_June_2011 7] [http://2011.igem.org/Team:Amsterdam/8_June_2011 8] [http://2011.igem.org/Team:Amsterdam/9_June_2011 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_June_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/12_June_2011&action=edit 12]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_June_2011&action=edit 13] [http://2011.igem.org/Team:Amsterdam/14_June_2011 14] [http://2011.igem.org/Team:Amsterdam/15_June_2011 15] [http://2011.igem.org/Team:Amsterdam/16_June_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_June_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_June_2011&action=edit 19]
[http://2011.igem.org/Team:Amsterdam/20_June_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_June_2011&action=edit 21] [http://2011.igem.org/Team:Amsterdam/22_June_2011 22] [http://2011.igem.org/Team:Amsterdam/23_June_2011 23] [http://2011.igem.org/Team:Amsterdam/24_June_2011 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_June_2011&action=edit 26]
[http://2011.igem.org/Team:Amsterdam/27_June_2011 27] [http://2011.igem.org/Team:Amsterdam/28_June_2011 28] [http://2011.igem.org/Team:Amsterdam/29_June_2011 29] [http://2011.igem.org/Team:Amsterdam/30_June_2011 30]
July
MTWTFSS
        [http://2011.igem.org/Team:Amsterdam/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_July_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_July_2011&action=edit 3]
[http://2011.igem.org/Team:Amsterdam/4_July_2011 4] [http://2011.igem.org/Team:Amsterdam/5_July_2011 5] [http://2011.igem.org/Team:Amsterdam/6_July_2011 6] [http://2011.igem.org/Team:Amsterdam/7_July_2011 7] [http://2011.igem.org/Team:Amsterdam/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_July_2011&action=edit 10]
[http://2011.igem.org/Team:Amsterdam/11_July_2011 11] [http://2011.igem.org/Team:Amsterdam/12_July_2011 12] [http://2011.igem.org/Team:Amsterdam/13_July_2011 13] [http://2011.igem.org/Team:Amsterdam/14_July_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_July_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_July_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_July_2011&action=edit 17]
[http://2011.igem.org/Team:Amsterdam/18_July_2011 18] [http://2011.igem.org/Team:Amsterdam/19_July_2011 19] [http://2011.igem.org/Team:Amsterdam/20_July_2011 20] [http://2011.igem.org/Team:Amsterdam/21_July_2011 21] [http://2011.igem.org/Team:Amsterdam/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_July_2011&action=edit 24]
[http://2011.igem.org/Team:Amsterdam/25_July_2011 25] [http://2011.igem.org/Team:Amsterdam/26_July_2011 26] [http://2011.igem.org/Team:Amsterdam/27_July_2011 27] [http://2011.igem.org/Team:Amsterdam/28_July_2011 28] [http://2011.igem.org/Team:Amsterdam/29_July_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_August_2011&action=edit 1] [http://2011.igem.org/Team:Amsterdam/2_August_2011 2] [http://2011.igem.org/Team:Amsterdam/3_August_2011 3] [http://2011.igem.org/Team:Amsterdam/4_August_2011 4] [http://2011.igem.org/Team:Amsterdam/5_August_2011 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_August_2011&action=edit 7]
[http://2011.igem.org/Team:Amsterdam/8_August_2011 8] [http://2011.igem.org/Team:Amsterdam/9_August_2011 9] [http://2011.igem.org/Team:Amsterdam/10_August_2011 10] [http://2011.igem.org/Team:Amsterdam/11_August_2011 11] [http://2011.igem.org/Team:Amsterdam/12_August_2011 12] [http://2011.igem.org/Team:Amsterdam/13_August_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_August_2011&action=edit 14]
[http://2011.igem.org/Team:Amsterdam/15_August_2011 15] [http://2011.igem.org/Team:Amsterdam/16_August_2011 16] [http://2011.igem.org/Team:Amsterdam/17_August_2011 17] [http://2011.igem.org/Team:Amsterdam/18_August_2011 18] [http://2011.igem.org/Team:Amsterdam/19_August_2011 19] [http://2011.igem.org/Team:Amsterdam/20_August_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_August_2011&action=edit 21]
[http://2011.igem.org/Team:Amsterdam/22_August_2011 22] [http://2011.igem.org/Team:Amsterdam/23_August_2011 23] [http://2011.igem.org/Team:Amsterdam/24_August_2011 24] [http://2011.igem.org/Team:Amsterdam/25_August_2011 25] [http://2011.igem.org/Team:Amsterdam/26_August_2011 26] [http://2011.igem.org/Team:Amsterdam/27_August_2011 27] [http://2011.igem.org/Team:Amsterdam/28_August_2011 28]
[http://2011.igem.org/Team:Amsterdam/29_August_2011 29] [http://2011.igem.org/Team:Amsterdam/30_August_2011 30] [http://2011.igem.org/Team:Amsterdam/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:Amsterdam/1_September_2011 1] [http://2011.igem.org/Team:Amsterdam/2_September_2011 2] [http://2011.igem.org/Team:Amsterdam/3_September_2011 3] [http://2011.igem.org/Team:Amsterdam/4_September_2011 4]
[http://2011.igem.org/Team:Amsterdam/5_September_2011 5] [http://2011.igem.org/Team:Amsterdam/6_September_2011 6] [http://2011.igem.org/Team:Amsterdam/7_September_2011 7] [http://2011.igem.org/Team:Amsterdam/8_September_2011 8] [http://2011.igem.org/Team:Amsterdam/9_September_2011 9] [http://2011.igem.org/Team:Amsterdam/10_September_2011 10] [http://2011.igem.org/Team:Amsterdam/11_September_2011 11]
[http://2011.igem.org/Team:Amsterdam/12_September_2011 12] [http://2011.igem.org/Team:Amsterdam/13_September_2011 13] [http://2011.igem.org/Team:Amsterdam/14_September_2011 14] [http://2011.igem.org/Team:Amsterdam/15_September_2011 15] [http://2011.igem.org/Team:Amsterdam/16_September_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_September_2011&action=edit 17] [http://2011.igem.org/Team:Amsterdam/18_September_2011 18]
[http://2011.igem.org/Team:Amsterdam/19_September_2011 19] [http://2011.igem.org/Team:Amsterdam/20_September_2011 20] [http://2011.igem.org/Team:Amsterdam/21_September_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_September_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_September_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_September_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_September_2011&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_September_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_September_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_September_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_September_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/1_October_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/2_October_2011&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:Amsterdam/31_October_2011&action=edit 31]


06-06-2011

Ideas for modelling

Brainstorm session with Douwe Molenaar and Isoude Kuijper

Steady state model of promoters

  • Information needed for model
    • Stability
      • Transcription
        • Measuring with qPCR
      • Translation
        • Fluorescence
    • Growth rate
  • Information needed for all promoters
    • Inducible promoter
      • IPTG promoter is also dependable on a lactose receptor/transporter
    • Cold shock promoter
  • Temperature dependent
    • The inducible promoters are also concentration dependent
      • Need to look into literature for already available information

The cold chock promoter

  • How does this promoter work?

Cryobrick

  • What concentration is needed?
    • How many targets do the chaperone proteins have?

Enzyme activity

  • Enzyme activity is also temperature dependent

Lab Work

  • Made a lot of plates and prepared Bba_R0010 and BBa_B0034 for miniprep testing tomorrow

Retrieved from "http://2011.igem.org/Team:Amsterdam/6_July_2011"