"
Team:Amsterdam/Notebook/Protocols/Making electrocompetent Cells
From 2011.igem.org
(Difference between revisions)
(→Preparation of electrocompetent cells) |
(→Preparation of electrocompetent cells) |
||
| Line 8: | Line 8: | ||
===Procedure=== | ===Procedure=== | ||
| - | *Prepare TOP10 preculture by inoculating 3ml of LB medium with a - | + | *Prepare TOP10 preculture by inoculating 3ml of LB medium with a -80°C TOP10 glycerol stock into and shake overnight at 37°C |
* | * | ||
Revision as of 08:34, 21 September 2011
Contents |
Preparation of electrocompetent cells
Overview
We use a protocol commonly used in our host lab provided by Diewertje Piebes.
Materials
Procedure
- Prepare TOP10 preculture by inoculating 3ml of LB medium with a -80°C TOP10 glycerol stock into and shake overnight at 37°C
