Team:Brown-Stanford/Lab/Protocols/BG11

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BG-11

(Taken from [http://microbiology.ucdavis.edu/meeks/BG11medium.html UC Davis])

Stock solutions for BG-11

Stock 1

0.1g/liter Na₂MG EDTA
0.6g/liter Ferric ammonium citrate
0.6g/liter Citric acid•1H₂O
3.6g/liter CaCl₂•2H₂O

Filter sterilize into a sterile bottle or autoclave.

Stock 2

7.5g/liter MgSO₄•7H₂O

Filter sterilize into a sterile bottle or autoclave.

Stock 3

4.0g/liter K_2HPO4•3H₂O
or 3.05g/liter K₂HPO₄

Filter sterilize into a sterile bottle or autoclave.

Stock 5 (Microelements)

Stock 5 (Microelements):

2.86g/liter H₃BO₃
1.81g/liter MnCl₂•4H₂O
0.222g/liter ZnSO₄•7H₂O
0.079g/liter CuSO₄•5H₂O
0.050g/liter COCl₂•6H₂O
0.391g/liter NaMoO₄•2H₂O
or 0.018g/liter MoO₄ (85%)

Basic BG11 medium

Combine the following stock solutions (for 1 liter):

10 mL Stock 1
10 mL Stock 2
10 mL Stock 3
0.02 g Na₂CO₃
1.0 mL Stock 5
1.5 g NaNO₃

Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 mL/125 mL flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO₃.

@@ Line 5: / Line 5: @@
 === '''Stock solutions for BG-11''' ===
-==== Stock 1: ====
+==== '''Stock 1''' ====
 *0.1g/liter Na<sub>2</sub>MG EDTA
 *0.6g/liter Ferric ammonium citrate
@@ Line 12: / Line 12: @@
 Filter sterilize into a sterile bottle or autoclave.
-Stock 2:
+==== '''Stock 2''' ====
 *7.5g/liter MgSO<sub>4</sub>&#8226;7H<sub>2</sub>O
 Filter sterilize into a sterile bottle or autoclave.
-Stock 3:
+==== '''Stock 3''' ====
 *4.0g/liter K<sub>2HPO</sub>4&#8226;3H<sub>2</sub>O
 *or 3.05g/liter K<sub>2</sub>HPO<sub>4</sub>
 Filter sterilize into a sterile bottle or autoclave.
+==== '''Stock 5 (Microelements)''' ====
 Stock 5 (Microelements):
 *2.86g/liter H<sub>3</sub>BO<sub>3</sub>
@@ Line 31: / Line 32: @@
-B) For basic BG11 medium combine the following stock solutions:
+=== '''Basic BG11 medium''' ===
-Stock Solution	Per Liter of medium
+Combine the following stock solutions (for 1 liter):
 *10 mL Stock 1
 *10 mL Stock 2
@@ Line 40: / Line 41: @@
 *1.5 g NaNO<sub>3</sub>
-Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO<sub>3</sub>.
+Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 mL/125 mL flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO<sub>3</sub>.
 {{:Team:Brown-Stanford/Templates/Foot}}