Team:British Columbia/Notebook/6 July 2011

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====beta-pinene====
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==July 06 2011==
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<b>beta-pinene</b>
*Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful.
*Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful.
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<p><b>(-)-limonene</b></p>
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*Gel verification of the SDM product shows some lanes with smears, and another with 2 bands suggesting unspecified annealing of primers. SDM failed.
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*Troubleshooting: DMSO could be lowering the Tm of the primers; Repeat the SDM PCR without adding DMSO for one sample, without adding MgCl<sub>2</sub> for another sample. Use the ramping cycling condition previously indicated.
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**Gel verification of the SDM product shows no bands. Addition of DMSO and MgCl<sub>2</sub> is not the problem.
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<b>1,8-Cineole</b>
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*Ran another SDM on pg-tps-cin, this time with more PFU. The gel suggests that it worked! Jacob then transformed E. coli with this product.

Latest revision as of 20:33, 30 July 2011

Team: British Columbia - 2011.igem.org

July 06 2011

beta-pinene

  • Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful.

(-)-limonene

  • Gel verification of the SDM product shows some lanes with smears, and another with 2 bands suggesting unspecified annealing of primers. SDM failed.
  • Troubleshooting: DMSO could be lowering the Tm of the primers; Repeat the SDM PCR without adding DMSO for one sample, without adding MgCl2 for another sample. Use the ramping cycling condition previously indicated.
    • Gel verification of the SDM product shows no bands. Addition of DMSO and MgCl2 is not the problem.

1,8-Cineole

  • Ran another SDM on pg-tps-cin, this time with more PFU. The gel suggests that it worked! Jacob then transformed E. coli with this product.
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