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Team:Brown-Stanford/Lab/Protocols/Bang
From 2011.igem.org
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{{:Team:Brown-Stanford/Templates/Protocol}} | {{:Team:Brown-Stanford/Templates/Protocol}} | ||
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<html><h1>Protocol Code: M7</h1></html> | <html><h1>Protocol Code: M7</h1></html> | ||
== '''Bang Media''' == | == '''Bang Media''' == | ||
| - | == '''By Percentage''' == | + | === '''By Percentage''' === |
*1% tryptone | *1% tryptone | ||
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*1% urea | *1% urea | ||
| - | == '''By Mass per Liter''' == | + | === '''By Mass per Liter''' === |
*10g tryptone | *10g tryptone | ||
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*'''Optional''': 15g agar (for plates) | *'''Optional''': 15g agar (for plates) | ||
| - | Mix in 200mL of water (without agar) to make a 5x solution. | + | === '''Notes''' === |
| + | |||
| + | *Mix in 200mL of water (without agar) to make a 5x solution. | ||
| + | *Needs to be at a pH of 8.5 | ||
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Latest revision as of 18:17, 28 September 2011
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REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
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Lab
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Partners
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Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocol Code: M7
Bang Media
By Percentage
- 1% tryptone
- 0.5% yeast extract
- 0.45 tricine
- 0.5% (NH4)2SO4
- 0.2% glutamic acid
- 1% urea
By Mass per Liter
- 10g tryptone
- 5g yeast extract
- 4.5g tricine
- 5g (NH4)2SO4
- 2g glutamic acid
- 10g urea
- Optional: 15g agar (for plates)
Notes
- Mix in 200mL of water (without agar) to make a 5x solution.
- Needs to be at a pH of 8.5
