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Team:Brown-Stanford/Lab/Protocols/SOB
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Latest revision as of 18:17, 28 September 2011
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Protocol Code: M6
SOB
Composition:
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter of SOB:
- 5 g yeast extract
- 20 g tryptone
- 10 mL NaCl (1M stock)
- 2.5 mL KCl (1M stock)
- 20 mL MgSO4 (1M stock)
