Team:Amsterdam/11 May 2011
From 2011.igem.org
(Difference between revisions)
Pvandieken (Talk | contribs) |
B.Stringer (Talk | contribs) m (moved Amsterdam/11 May 2011 to Team:Amsterdam/11 May 2011) |
||
(4 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
{{:Team:Amsterdam/Header}} | {{:Team:Amsterdam/Header}} | ||
{{:Team:Amsterdam/Notebook/Calendar}} | {{:Team:Amsterdam/Notebook/Calendar}} | ||
- | = | + | =11-05-2011 | VU Meeting for topics discussion= |
- | + | ==Ethylene producing ''E.coli''== | |
*The project is to short | *The project is to short | ||
*Are there alternative ways to meassure ethylene? | *Are there alternative ways to meassure ethylene? | ||
Line 9: | Line 9: | ||
*Evolution doesn't create biobricks | *Evolution doesn't create biobricks | ||
- | === | + | ===Neglected disease=== |
- | + | ====Kala-azar==== | |
*Is the protein available? | *Is the protein available? | ||
*Whole parasite is a problem | *Whole parasite is a problem | ||
Line 19: | Line 19: | ||
**Target was an excreted protein | **Target was an excreted protein | ||
- | = | + | ====Hookworm==== |
*CCL11 will be cut and luciferase fades when the hookworm is in the sample | *CCL11 will be cut and luciferase fades when the hookworm is in the sample | ||
**Can't we do this the other way around | **Can't we do this the other way around | ||
Line 26: | Line 26: | ||
*Are CCL11 and metalloproteases available for in the lab? | *Are CCL11 and metalloproteases available for in the lab? | ||
- | = | + | ====Buruli ulcer==== |
*It is a bacteria | *It is a bacteria | ||
*It is checked with FRET | *It is checked with FRET |
Latest revision as of 10:28, 21 September 2011
|
|
|
|
|
|
|
11-05-2011 | VU Meeting for topics discussion
Ethylene producing E.coli
- The project is to short
- Are there alternative ways to meassure ethylene?
- Use colors to check if ethylene is produced
- Ethylene sensing is difficult, because of the receptor.
- Evolution doesn't create biobricks
Neglected disease
Kala-azar
- Is the protein available?
- Whole parasite is a problem
- Signal transduction via peanut agglutin can't be done
- Check if peanut agglutin binds to other stuff in human body fluids
- We need specialists on this subject
- Look at Imperial college, they've done a project on parasites
- Target was an excreted protein
Hookworm
- CCL11 will be cut and luciferase fades when the hookworm is in the sample
- Can't we do this the other way around
- Metalloproteases cleaves the CCl11, if this isn't available it doesn't promote luciferase production
- Can this be done in E.coli
- Are CCL11 and metalloproteases available for in the lab?
Buruli ulcer
- It is a bacteria
- It is checked with FRET
- Can the specific receptor be palced in E.coli
- The pathogen is known, but no detection method yet
- Can mycolactone be detected?
- Membrane receptor can be a problem
- Maybe use Mycobacteria