Team:Amsterdam/6 July 2011
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+ | {{:Team:Amsterdam/Header}} | ||
+ | {{:Team:Amsterdam/Notebook/Calendar}} | ||
+ | = 06-07-2011 = | ||
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=Ideas for modelling= | =Ideas for modelling= | ||
''Brainstorm session with Douwe Molenaar and Isoude Kuijper'' | ''Brainstorm session with Douwe Molenaar and Isoude Kuijper'' | ||
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***Transcription | ***Transcription | ||
****Measuring with qPCR | ****Measuring with qPCR | ||
- | **Translation | + | ***Translation |
- | ***Fluorescence | + | ****Fluorescence |
**Growth rate | **Growth rate | ||
*Information needed for all promoters | *Information needed for all promoters | ||
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** The inducible promoters are also concentration dependent | ** The inducible promoters are also concentration dependent | ||
*** Need to look into literature for already available information | *** Need to look into literature for already available information | ||
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==The cold chock promoter== | ==The cold chock promoter== | ||
*How does this promoter work? | *How does this promoter work? | ||
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==Enzyme activity== | ==Enzyme activity== | ||
* Enzyme activity is also temperature dependent | * Enzyme activity is also temperature dependent | ||
+ | =Lab Work= | ||
+ | *Made a lot of plates and prepared Bba_R0010 and BBa_B0034 for miniprep testing tomorrow | ||
+ | {{:Team:Amsterdam/Footer}} |
Latest revision as of 10:30, 21 September 2011
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06-07-2011
Ideas for modelling
Brainstorm session with Douwe Molenaar and Isoude Kuijper
Steady state model of promoters
- Information needed for model
- Stability
- Transcription
- Measuring with qPCR
- Translation
- Fluorescence
- Transcription
- Growth rate
- Stability
- Information needed for all promoters
- Inducible promoter
- IPTG promoter is also dependable on a lactose receptor/transporter
- Cold shock promoter
- Inducible promoter
- Temperature dependent
- The inducible promoters are also concentration dependent
- Need to look into literature for already available information
- The inducible promoters are also concentration dependent
The cold chock promoter
- How does this promoter work?
Cryobrick
- What concentration is needed?
- How many targets do the chaperone proteins have?
Enzyme activity
- Enzyme activity is also temperature dependent
Lab Work
- Made a lot of plates and prepared Bba_R0010 and BBa_B0034 for miniprep testing tomorrow