Team:Brown-Stanford/Lab/Protocols/Nutrient

From 2011.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Brown-Stanford/Templates/Protocol}}
{{:Team:Brown-Stanford/Templates/Protocol}}
 +
<html><h1>Protocol Code: M5</h1></html>
== '''Nutrient agar with urea''' ==
== '''Nutrient agar with urea''' ==
 +
 +
*0.5% Peptone
*0.5% Peptone
*0.3% beef extract/yeast extract
*0.3% beef extract/yeast extract
Line 9: Line 12:
*pH adjusted to neutral (6.8)at 25 °C.
*pH adjusted to neutral (6.8)at 25 °C.
*8% urea that is filter sterilized, added after the rest of the stuff is autoclaved
*8% urea that is filter sterilized, added after the rest of the stuff is autoclaved
-
 
{{:Team:Brown-Stanford/Templates/Foot}}
{{:Team:Brown-Stanford/Templates/Foot}}

Latest revision as of 18:17, 28 September 2011

Brown-Stanford
iGEM

Protocol Code: M5

Nutrient agar with urea

  • 0.5% Peptone
  • 0.3% beef extract/yeast extract
  • 1.5% agar
  • 85.8 mM NaCl
  • distilled water
  • pH adjusted to neutral (6.8)at 25 °C.
  • 8% urea that is filter sterilized, added after the rest of the stuff is autoclaved