Team:Fatih Turkey/Experiments2

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<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
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<ul>
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li>
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li>
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
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href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li>
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             <ol>
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               <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Confirmation</a></li>
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               <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li>
                
                
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                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li>
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                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
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                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li>
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                  <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
                   <li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li>
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li>
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            <p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p>
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<table width="606" height="227" border="0">
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  <tr>
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    <td width="600" height="223" valign="top"><p><strong>THE SUICIDE EXPERIMENT OF E. COLI</strong><br />
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<p><strong><img src="https://static.igem.org/mediawiki/2011/e/e7/DSC03845.JPG" width="500" /></strong></p>
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          <em>Our K541545 part contains IPTG promoter and the gene  part of LALF protein on a backbone which has resistance to chloramphenicol. We  transported this part to E. coli for testing whether E. coli would stop its own  growth like a suicide.</em> <br />
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          <strong>Assays of plates with and without IPTG</strong> <br />
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          <strong>Assay 1:</strong> We streaked E. coli culture which has BBa_K541545.  Media also included chloramphenicol and IPTG. As a control group, a plate that  has no IPTG is prepared. We aimed to see less colonies comparing with control  group. <br />
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          <strong>Assay 2:</strong> This experiment was done in order to eliminate the  possibility of toxic effect of IPTG. E.coli without IPTG promoter was streaked  a plate that does not have IPTG. For a second plate, E.coli with PlacI promoter  was added into plate with IPTG.</p></td>
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Latest revision as of 20:07, 28 October 2011

deneme baslik

Experiments

The Suicide Experiment of E.Coli

THE SUICIDE EXPERIMENT OF E. COLI


Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would stop its own growth like a suicide.
Assays of plates with and without IPTG
Assay 1: We streaked E. coli culture which has BBa_K541545. Media also included chloramphenicol and IPTG. As a control group, a plate that has no IPTG is prepared. We aimed to see less colonies comparing with control group.
Assay 2: This experiment was done in order to eliminate the possibility of toxic effect of IPTG. E.coli without IPTG promoter was streaked a plate that does not have IPTG. For a second plate, E.coli with PlacI promoter was added into plate with IPTG.

2011 © Fatih Medical School

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