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Team:Brown-Stanford/Lab/Protocols/BG11
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<html><h1>Protocol Code: M1</h1></html> | <html><h1>Protocol Code: M1</h1></html> | ||
Latest revision as of 18:15, 28 September 2011
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Protocol Code: M1
BG-11
(Taken from Meeks Lab at University of California, Davis)
Stock solutions for BG-11
Stock 1
- 0.1g/liter Na2MG EDTA
- 0.6g/liter Ferric ammonium citrate
- 0.6g/liter Citric acid•1H2O
- 3.6g/liter CaCl2•2H2O
Filter sterilize into a sterile bottle or autoclave.
Stock 2
- 7.5g/liter MgSO4•7H2O
Filter sterilize into a sterile bottle or autoclave.
Stock 3
- 4.0g/liter K2HPO4•3H2O or 3.05g/liter K2HPO4
Filter sterilize into a sterile bottle or autoclave.
Stock 5 (Microelements)
Stock 5 (Microelements):
- 2.86g/liter H3BO3
- 1.81g/liter MnCl2•4H2O
- 0.222g/liter ZnSO4•7H2O
- 0.079g/liter CuSO4•5H2O
- 0.050g/liter COCl2•6H2O
- 0.391g/liter NaMoO4•2H2O or 0.018g/liter MoO4 (85%)
Basic BG11 medium
Combine the following stock solutions (for 1 liter):
- 10 mL Stock 1
- 10 mL Stock 2
- 10 mL Stock 3
- 0.02 g Na2CO3
- 1.0 mL Stock 5
- 1.5 g NaNO3
Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 mL/125 mL flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO3.
