Team:Brown-Stanford/Lab/Protocols/CementationColumn

From 2011.igem.org

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== '''Biocementation Column''' ==
== '''Biocementation Column''' ==
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=== '''Materials''' ===
=== '''Materials''' ===
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*60 mL falcon tube  
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*15 mL falcon tube  
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*30 g of Lunar/Martian regolith per tube
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*20 g of Lunar/Martian regolith per tube
*A peristaltic pump  
*A peristaltic pump  
*Two feet of rubber tubing, epoxy glue, fabric matting
*Two feet of rubber tubing, epoxy glue, fabric matting
*Heat Lamp
*Heat Lamp
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*50 mL of S. pasteurii culture (OD600 1.2)
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*30 mL of S. pasteurii culture (OD600 1.2)
=== '''Procedure''' ===
=== '''Procedure''' ===

Latest revision as of 18:17, 28 September 2011

Brown-Stanford
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Biocementation Column

Materials

  • 15 mL falcon tube
  • 20 g of Lunar/Martian regolith per tube
  • A peristaltic pump
  • Two feet of rubber tubing, epoxy glue, fabric matting
  • Heat Lamp
  • 30 mL of S. pasteurii culture (OD600 1.2)

Procedure

  • The columns were first prewet with 20 mL of sterile Bang media.
  • The bacteria culture was draw into the feed tube, and allowed to settle in the column overnight
  • Cementation solution (1.5 M equimolar urea and calcium chloride) was fed into the feed tube, which was then placed into a constant loop with the output tube
  • The column was heated for 5 days, and allowed to cycle.

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