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Team:British Columbia/Notebook/11 July 2011
From 2011.igem.org
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(Created page with "====beta-pinene==== *Transformation plates taken out. *All 3 plates (P2SDM1, P2SDM2, P2SDM3) have colonies. *Set up overnight culture for miniprepping.") |
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| + | <b>beta-pinene</b> | ||
*Transformation plates taken out. | *Transformation plates taken out. | ||
*All 3 plates (P2SDM1, P2SDM2, P2SDM3) have colonies. | *All 3 plates (P2SDM1, P2SDM2, P2SDM3) have colonies. | ||
*Set up overnight culture for miniprepping. | *Set up overnight culture for miniprepping. | ||
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| + | <b>(-)-limonene</b> | ||
| + | *SDM PCR has failed thus far. | ||
| + | *Troubleshooting: Use less pfu as the glycerol content in the enzyme could alter the reaction - prepared 3 samples, each with 0.5uL pfu, 1ul pfu, and 2ul pfu, respectively. Also prepare a sample using 0.5 taq polymerase only. Also increased the annealing temperature and elongation time. | ||
Revision as of 17:15, 20 July 2011
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beta-pinene
- Transformation plates taken out.
- All 3 plates (P2SDM1, P2SDM2, P2SDM3) have colonies.
- Set up overnight culture for miniprepping.
(-)-limonene
- SDM PCR has failed thus far.
- Troubleshooting: Use less pfu as the glycerol content in the enzyme could alter the reaction - prepared 3 samples, each with 0.5uL pfu, 1ul pfu, and 2ul pfu, respectively. Also prepare a sample using 0.5 taq polymerase only. Also increased the annealing temperature and elongation time.
