Team:British Columbia/Notebook/11 July 2011

From 2011.igem.org

Team: British Columbia - 2011.igem.org

July 11 2011

beta-pinene

  • Transformation plates taken out.
  • All 3 plates (P2SDM1, P2SDM2, P2SDM3) have colonies.
  • Set up overnight culture for miniprepping.

(-)-limonene

  • SDM PCR has failed thus far.
  • Troubleshooting: Use less pfu as the glycerol content in the enzyme could alter the reaction - prepared 3 samples, each with 0.5uL pfu, 1ul pfu, and 2ul pfu, respectively. Also prepare a sample using 0.5 taq polymerase only. Also increased the annealing temperature and elongation time.