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Team:British Columbia/Notebook/6 July 2011
From 2011.igem.org
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*Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful. | *Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful. | ||
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| + | <p><b>(-)-limonene</b></p> | ||
| + | *Gel verification of the SDM product shows some lanes with smears, and another with 2 bands suggesting unspecified annealing of primers. SDM failed. | ||
| + | *Troubleshooting: DMSO could be lowering the Tm of the primers; Repeat the SDM PCR without adding DMSO for one sample, without adding MgCl<sub>2</sub> for another sample. Use the ramping cycling condition previously indicated. | ||
| + | **Gel verification of the SDM product shows no bands. Addition of DMSO and MgCl<sub>2</sub> is not the problem. | ||
Revision as of 17:12, 20 July 2011
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beta-pinene
- Gel verification of the SDM product shows bands of the correct size suggesting that the SDM was successful.
(-)-limonene
- Gel verification of the SDM product shows some lanes with smears, and another with 2 bands suggesting unspecified annealing of primers. SDM failed.
- Troubleshooting: DMSO could be lowering the Tm of the primers; Repeat the SDM PCR without adding DMSO for one sample, without adding MgCl2 for another sample. Use the ramping cycling condition previously indicated.
- Gel verification of the SDM product shows no bands. Addition of DMSO and MgCl2 is not the problem.
