Team:Brown-Stanford/Lab/Protocols/Bang

From 2011.igem.org

(Difference between revisions)
(By Mass per Liter)
(Bang Media)
Line 27: Line 27:
*Mix in 200mL of water (without agar) to make a 5x solution.
*Mix in 200mL of water (without agar) to make a 5x solution.
-
*Needs to be at a pH of 6.8
+
*Needs to be at a pH of 8.5
{{:Team:Brown-Stanford/Templates/Foot}}
{{:Team:Brown-Stanford/Templates/Foot}}

Revision as of 18:22, 18 July 2011

Brown-Stanford
iGEM

Protocol Code: M7

Bang Media

By Percentage

  • 1% tryptone
  • 0.5% yeast extract
  • 0.45 tricine
  • 0.5% (NH4)2SO4
  • 0.2% glutamic acid
  • 1% urea

By Mass per Liter

  • 10g tryptone
  • 5g yeast extract
  • 4.5g tricine
  • 5g (NH4)2SO4
  • 2g glutamic acid
  • 10g urea
  • Optional: 15g agar (for plates)

Notes

  • Mix in 200mL of water (without agar) to make a 5x solution.
  • Needs to be at a pH of 8.5