Team:Brown-Stanford/Lab/Protocols/Bang
From 2011.igem.org
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*'''Optional''': 15g agar (for plates) | *'''Optional''': 15g agar (for plates) | ||
- | Mix in 200mL of water (without agar) to make a 5x solution. | + | === '''Notes''' === |
+ | |||
+ | *Mix in 200mL of water (without agar) to make a 5x solution. | ||
+ | *Needs to be at a pH of 6.8 | ||
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} |
Revision as of 19:00, 13 July 2011
Protocol Code: M7
Bang Media
By Percentage
- 1% tryptone
- 0.5% yeast extract
- 0.45 tricine
- 0.5% (NH4)2SO4
- 0.2% glutamic acid
- 1% urea
By Mass per Liter
- 10g tryptone
- 5g yeast extract
- 4.5g tricine
- 5g (NH4)2SO4
- 2g glutamic acid
- 10g urea
- Optional: 15g agar (for plates)
Notes
- Mix in 200mL of water (without agar) to make a 5x solution.
- Needs to be at a pH of 6.8